recombinant full length murine angptl4 Search Results


recombinant full length murine angptl4  (R&D Systems)
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R&D Systems recombinant full length murine angptl4
FIGURE 5 Lipolysis products induce the expression of <t>Angptl4</t> in MC3T3‐E1 cells. Cells were exposed to 0−150 mg/dL TGRL mixed with 2 units/mL LPL for 3 or 6 h. Bars represent mean ± SEM, n = 4−6, ****p < 0.0001 compared to 0 TGRL + LPL (Ctrl). LPL, lipoprotein lipase; TGRL, triglyceride‐rich lipoproteins.
Recombinant Full Length Murine Angptl4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant full length murine angptl4 - by Bioz Stars, 2026-10
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FIGURE 5 Lipolysis products induce the expression of Angptl4 in MC3T3‐E1 cells. Cells were exposed to 0−150 mg/dL TGRL mixed with 2 units/mL LPL for 3 or 6 h. Bars represent mean ± SEM, n = 4−6, ****p < 0.0001 compared to 0 TGRL + LPL (Ctrl). LPL, lipoprotein lipase; TGRL, triglyceride‐rich lipoproteins.

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Lipolysis products from triglyceride-rich lipoproteins induce stress protein ATF3 in osteoblasts.

doi: 10.1002/jor.25756

Figure Lengend Snippet: FIGURE 5 Lipolysis products induce the expression of Angptl4 in MC3T3‐E1 cells. Cells were exposed to 0−150 mg/dL TGRL mixed with 2 units/mL LPL for 3 or 6 h. Bars represent mean ± SEM, n = 4−6, ****p < 0.0001 compared to 0 TGRL + LPL (Ctrl). LPL, lipoprotein lipase; TGRL, triglyceride‐rich lipoproteins.

Article Snippet: Recombinant full length murine ANGPTL4 (4880‐AN‐050) was purchased from R&D Systems.

Techniques: Expressing

FIGURE 6 Recombinant ANGPTL4 inhibits lipolysis product‐ induction of Atf3 in MC3T3‐E1 cells. Cells were exposed to 100 mg/dL TGRL mixed with 2 units/mL LPL for 6 h. In some experiments, LPL and ANGPTL4 (0.1−5 µg/mL) were mixed for 30 min before the addition of TGRL. Ctrl represents cells not exposed to TGRL/LPL/ANGPTL4. Bars represent mean ± SEM, n = 4−6. *p < 0.05, ***p < 0.001, ****p < 0.0001 compared to 0 µg/mL ANGPTL4. LPL, lipoprotein lipase; TGRL, triglyceride‐rich lipoproteins.

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Lipolysis products from triglyceride-rich lipoproteins induce stress protein ATF3 in osteoblasts.

doi: 10.1002/jor.25756

Figure Lengend Snippet: FIGURE 6 Recombinant ANGPTL4 inhibits lipolysis product‐ induction of Atf3 in MC3T3‐E1 cells. Cells were exposed to 100 mg/dL TGRL mixed with 2 units/mL LPL for 6 h. In some experiments, LPL and ANGPTL4 (0.1−5 µg/mL) were mixed for 30 min before the addition of TGRL. Ctrl represents cells not exposed to TGRL/LPL/ANGPTL4. Bars represent mean ± SEM, n = 4−6. *p < 0.05, ***p < 0.001, ****p < 0.0001 compared to 0 µg/mL ANGPTL4. LPL, lipoprotein lipase; TGRL, triglyceride‐rich lipoproteins.

Article Snippet: Recombinant full length murine ANGPTL4 (4880‐AN‐050) was purchased from R&D Systems.

Techniques: Recombinant

FIGURE 11 (A) Lipoprotein lipase (LPL) (or hepatic lipase) secreted by the osteoblast translocates to the plasma membrane of endothelial cells lining blood vessels, where it catalyzes the conversion of triglyceride‐rich lipoproteins (TGRL) into lipolysis products (LP) such as free fatty acids (FFA). LP translocate to the osteoblast for use as a metabolic fuel source. (B) High circulating levels of TGRL result in high levels of LP which induce an increase in ATF3 in osteoblasts (pathway 1), and induce IL6 and COX‐2, known downstream genes of ATF3; LP also induce intracellular ROS accumulation, which together result in inflammation. LP induce an increase in angiopoietin‐like 4 (ANGPTL4) (pathway 2), an inhibitor of LPL, which may act in a negative feedback loop, reducing LPL activity, reducing LP accumulation in the local environment, and protecting cells from inflammation. Note it is possible that lipases may be active on the osteoblast cell membrane itself. ATF3, activating transcription factor 3; ROS, reactive oxygen species.

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Lipolysis products from triglyceride-rich lipoproteins induce stress protein ATF3 in osteoblasts.

doi: 10.1002/jor.25756

Figure Lengend Snippet: FIGURE 11 (A) Lipoprotein lipase (LPL) (or hepatic lipase) secreted by the osteoblast translocates to the plasma membrane of endothelial cells lining blood vessels, where it catalyzes the conversion of triglyceride‐rich lipoproteins (TGRL) into lipolysis products (LP) such as free fatty acids (FFA). LP translocate to the osteoblast for use as a metabolic fuel source. (B) High circulating levels of TGRL result in high levels of LP which induce an increase in ATF3 in osteoblasts (pathway 1), and induce IL6 and COX‐2, known downstream genes of ATF3; LP also induce intracellular ROS accumulation, which together result in inflammation. LP induce an increase in angiopoietin‐like 4 (ANGPTL4) (pathway 2), an inhibitor of LPL, which may act in a negative feedback loop, reducing LPL activity, reducing LP accumulation in the local environment, and protecting cells from inflammation. Note it is possible that lipases may be active on the osteoblast cell membrane itself. ATF3, activating transcription factor 3; ROS, reactive oxygen species.

Article Snippet: Recombinant full length murine ANGPTL4 (4880‐AN‐050) was purchased from R&D Systems.

Techniques: Clinical Proteomics, Membrane, Activity Assay